Home > Sandwich ELISA Kits > Mouse Platelet Derived Growth Factor Subunit B (PDGFB) ELISA Kit

Product Name:

Mouse Platelet Derived Growth Factor Subunit B (PDGFB) ELISA Kit

Cat.#:

JL45395

Regulatory Status:

RUO

Alternative:

PDGF-B; PDGF2; SIS; SSV; C-sis; Simian Sarcoma Viral(v-sis)Oncogene Homolog; Platelet Derived Growth Factor Beta Polypeptide; Proto-oncogene c-Sis; Becaplermin

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

31.25-2000pg/mL

Sensitivity:

14.23pg/mL

Specificity:

This kit recognizes Mouse PDGFB in samples. No significant cross-reactivity or interference between Mouse PDGFB and analogues was observed.

Sample Type:

Serum;plasma;tissue homogenates;cell lysates and other biological fluids

Assay Procedure:

Research Area:

Cell Biology

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| PDGFB

Platelet-derived growth factor subunit B is a protein that is encoded by the PDGFB gene.The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha (PDGFA) polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Mouse PDGFB spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	93-105	103
Cell Lysates(n=8)	96-108	105

| Linearity

Samples were spiked with high concentrations of Mouse PDGFB and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Lysates(n=4)
1 : 2	Range (%)	84-95	89-96	90-110
1 : 2	Average (%)	91	94	96
1 : 4	Range (%)	89-103	87-108	105-115
1 : 4	Average (%)	94	97	108